Table 3.
Kinetic constants (Km, μM; kcat, s−1; and kcat/Km, s−1 mM−1) of wild-type VP (allelic variant VPL2) and its E243Q variant oxidizing VA, DMP, ABTS and Mn2+ (means and 95% confidence limits).
| Wild-type VPL2 | E243Q variant | ||
|---|---|---|---|
| VA | Km | 2750.0 ± 50.0 | 2270.0 ± 150.0 |
| kcat | 6.6 ± 0.0 | 1.6 ± 0.0 | |
| kcat/Km | 2.4 ± 0.0 | 0.7 ± 0.0 | |
| DMP | Km | 18.0 ± 1.0 | 18.3 ± 1.3 |
| kcat | 7.1 ± 0.1 | 2.6 ± 0.0 | |
| kcat/Km | 403.0 ± 24.0 | 142.0 ± 10.0 | |
| ABTS | Km | 7.9 ± 0.9 | 7.3 ± 0.6 |
| kcat | 15.4 ± 0.6 | 7.8 ± 2.5 | |
| kcat/Km | 1950.0 ± 240.0 | 1070.0 ± 390.0 | |
| Mn2+ | Km | 268.0 ± 20.0 | 224.0 ± 10.0 |
| kcat | 282.1 ± 6.1 | 248.0 ± 3.0 | |
| kcat/Km | 1050.0 ± 80.0 | 1100.0 ± 50.0 |