Table 1.
Cytokine and chemokine profiling of hAFS secretome obtained following following 24h 1% O2 hypoxic preconditioning and compared to normoxic conditions (Ctrl hAFS-CM).
| Ctrl hAFS-CM | Hypoxic hAFS-CM | |
|---|---|---|
| PAI-1 | 1 | 0.90 ± 0.18 |
| IL-17α | 1 | 1.00 ± 0.36 |
| SDF-1α | 1 | 1.00 ± 0.51 |
| IL-11 | 1 | 1.29 ± 0.11 |
| MIF | 1 | 1.67 ± 0,49 |
| IL-8 | 1 | 2.39 ± 1.32 |
| IL-6 | 1 | 3.20 ± 2.41 |
| OPN | 1 | 3.55 ± 1.43 |
| FGF-19 | 1 | 3.58 ± 1.98 |
| MCP-1 | 1 | 4.23 ± 1.45 |
| GDF-15 | 1 | 8.87 ± 4.64 |
Values are assessed by quantification of positive pixels for each selected cytokine on the array membrane and are expressed as mean ± s. e.m of the fold change in the cytokine/chemokine expression of hypoxic hAFS secretome (Hypoxic hAFS-CM) over control normoxic hAFS-CM (Ctrl hAFS-CM) of n = 3 experiments; FGF-19: Fibroblast Growth Factor 19; GDF-15: Growth/differentiation factor 15; IL-6: Interleukin-6; IL-11: Interleukin-11; IL-17α: Interleukin- 17α: MCP-1: Monocyte Chemotactic Protein-1; MIF: Macrophage migration inhibitory factor; OPN: Osteopontin; SDF-1α: Stromal cell-Derived Factor 1-alpha; PAI-1: Plasminogen Activator Inhibitor-1.