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. 2019 Aug 13;10:1842. doi: 10.3389/fmicb.2019.01842

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Copyright © 2019 Nair, Sharan and Ajitkumar.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Determination of the oxidative status and ROS (hydroxyl radical and H₂O₂) levels in the unexposed freshly prepared SCF/Mrx1-roGFP2 and NCF/Mrx1-roGFP2 cells. Flow cytometry profile of roGFP2 fluorescence of: (A) SCF/Mrx1-roGFP2 cells, (D) NCF/Mrx1-roGFP2 cells. (B,E) Scatter plot of roGFP2 fluorescence of the respective cells from the data in (A,D), respectively. (C,F) Percentages of the respective cells in each quadrant with their V500 and FITC median fluorescence values and their ratios. The major proportion of the cells amongst the four quadrants and their fluorescence ratio are given in bold red color. (G) Quantitation of V500:FITC fluorescence ratio in the SCF/Mrx1-roGFP2 and NCF/Mrx1-roGFP2 cells (n = 3). Statistical significance was calculated using paired t-test where ^*Indicates p ≤ 0.05.