Figure 1.

CABLES1 Expression in Human and Murine Hematopoietic and Niche Cells

(A) Experimental strategy used to probe functions of CABLES1 in hematopoiesis. HSC, hematopoietic stem cells; shRNA, short hairpin RNA; 5-FU, 5-fluorouracil.

(B) Cables1 mRNA expression in mouse cells sorted by fluorescence-activated cell sorting: B cells (B220⁺), T cells (CD4⁺ and CD8⁺), myeloid (Gr-1⁺) cells, Lin⁻ (lineage marker-negative cells, namely CD3⁻B220⁻Ter119⁻Gr-1⁻), LSK (Lin⁻c-Kit⁺Sca-1⁺), c-kit⁺ (Lin⁻c-Kit⁺Sca-1⁻), SLAM (CD150⁺CD48⁻LSK), CMPs (Lin⁻Sca-1⁺c-Kit⁺FcR⁻CD34⁺), GMP (Lin⁻Sca-1⁺c-Kit⁺FcR⁺CD34⁺), MEP (Lin⁻Sca-1⁺c-Kit⁺FcR⁻CD34⁻); and in cellular components of the BM microenvironment: osteoblasts (CD45⁻Ter119⁻CD31⁻Sca-1⁻CD51⁺), endothelial cells (CD45⁻Ter119⁻CD31⁺), and MSCs (CD45⁻Ter119⁻CD31⁻Sca⁺CD51⁺). Data are normalized to HPRT transcript levels and mouse brain is used as reference. Data represent a pool from 10 mice and are the mean ± SEM of triplicates. See also Figure S1.

(C) CABLES1 expression in human CD34⁺ cells from cord blood (CB-CD34⁺), mobilized peripheral blood (PB-CD34), and mature blood cells (CD4⁺, CD8⁺, CD14⁺, CD19⁺, CD56⁺). Data are normalized to HPRT transcript levels and CD34⁺ cells are used as reference.

(D) Expression level of human CABLES1 in CB-CD34⁺, CD3⁺, CD14⁺, and MSCs by western blot. Two different MSC samples are shown as MSC1 and MSC2. See also Figure S2.

(E) Immunolocalization of CABLES1 in CB-CD34⁺ cells. Staining with the anti-CABLES1 antibody of human CD3⁺ cells that do not express significant levels of CABLES1 mRNA is presented in the lower panel.