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. 2019 Jul 25;13(2):366–379. doi: 10.1016/j.stemcr.2019.06.004

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© 2019 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

ROR1 Serves as Predictive Surrogate Marker for Cardiomyocyte Formation

(A) Volcano plot comparing cardiac and presomitic mesoderm on day 3 of differentiation. Key genes for each condition are highlighted in respective colors, either grouped in categories (, , , ) or individually (, ). Note the detection of ROR1 () in the cardiac mesoderm by three independent probes and the minor difference for established surface markers (), n = 4 independent experiments.

(B) Representative FC histograms for ROR2, CD13, and ROR1 on day 3 with () and without () IWP2 treatment on day 1.

(C) Gene expression in the gastrulating mouse embryo (Peng et al., 2016) indicates Ror1 expression (right panel) in the anterior-to-mid PS region whereby T expression (left panel) increases toward the posterior PS.

(D) Pattern of mesodermal surface markers in human fetal tissues (Kim et al., 2014) indicates high ROR1 levels in the heart.

(E) Representative FC on day 3 for NCAM/EpCAM, CD13/ROR2 and CD13/ROR1 with/without addition of IWP2 on day 1 and corresponding CM content (cTNT, SA, MHC) on day 10.

(F) Correlation of ROR1⁺/CD13⁺ on day 3 and respective CM content on day 10 based on three different cell lines; each dot represents an independent biological repeat. r denotes Pearson's correlation coefficient. Dotted lines denote 95% confidence interval.

See also Figure S2.

Inline graphic — ROR1 Serves as Predictive Surrogate Marker for Cardiomyocyte Formation

(A) Volcano plot comparing cardiac and presomitic mesoderm on day 3 of differentiation. Key genes for each condition are highlighted in respective colors, either grouped in categories (, , , ) or individually (, ). Note the detection of ROR1 () in the cardiac mesoderm by three independent probes and the minor difference for established surface markers (), n = 4 independent experiments.

(B) Representative FC histograms for ROR2, CD13, and ROR1 on day 3 with () and without () IWP2 treatment on day 1.

(C) Gene expression in the gastrulating mouse embryo (Peng et al., 2016) indicates Ror1 expression (right panel) in the anterior-to-mid PS region whereby T expression (left panel) increases toward the posterior PS.

(D) Pattern of mesodermal surface markers in human fetal tissues (Kim et al., 2014) indicates high ROR1 levels in the heart.

(E) Representative FC on day 3 for NCAM/EpCAM, CD13/ROR2 and CD13/ROR1 with/without addition of IWP2 on day 1 and corresponding CM content (cTNT, SA, MHC) on day 10.

(F) Correlation of ROR1⁺/CD13⁺ on day 3 and respective CM content on day 10 based on three different cell lines; each dot represents an independent biological repeat. r denotes Pearson's correlation coefficient. Dotted lines denote 95% confidence interval.

See also Figure S2.