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. 2019 Jul 25;13(2):366–379. doi: 10.1016/j.stemcr.2019.06.004

Figure 3.

Figure 3

Day-2 Aggregates Derived in E8 Show Superior CM Formation in Chemically Defined Differentiation Medium

(A) Experimental scheme for optimizing expansion timing before differentiation. Microscopy of day 2/day 4 aggregates.

(B) Diameter assessment: evaluating >100 aggregates on day 2/day 4 from three independent runs. p < 0.0001.

(C) Cell density on day 2/day 4 of three independent runs in duplicates, respectively. p = 0.0019.

(D) Representative pH/DO curves of preculture.

(E and F) The impact of CHIR concentration and preculture duration. FC on day 10 revealed higher content of GFP+ CMs applying day-2 precultures in (E) RPMI/B27 (n = 3 independent experiments, preculture duration p =0.0262 and 5 versus 7.5 μM CHIR p = 0.0439) or (F) CDM3 (n = 3 independent experiments, 7.5 versus 70 μM CHIR p = 0.0095).

Error bars represent SEM. See also Figure S3.