Process Upscaling in Stirred Tank Bioreactors Confirms Utility of CD13+/ROR1+ for Early Process Monitoring and Enables >90% CM Induction
(A) Scheme for differentiation in bioreactors and microscopic assessment of aggregates over time. Scale bars, 200 μm.
(B) Growth kinetics in DASbox (n = 3 independent experiments) and the Bioblock system (n = 3 independent experiments). Error bars represent SEM.
(C) Cardiac differentiation efficiency assessed by FC on day 10 for cTNT, SA, and MHC revealed >90% purity in DASbox and ∼90% in Bioblock (n = 3 independent experiments). Error bars represent SEM.
(D) Correlation of ROR1+/CD13+ on day 3 and respective CM content on day 10 of Phoenix in specific differentiation conditions resulting from upscale experiments. r denotes Pearson's correlation coefficient (n = 28 in seven independent experiments). Dotted line denotes 95% confidence interval.
See also Figure S4.