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. 2019 Jul 25;13(2):366–379. doi: 10.1016/j.stemcr.2019.06.004

Figure 5.

Figure 5

Process Upscaling in Stirred Tank Bioreactors Confirms Utility of CD13+/ROR1+ for Early Process Monitoring and Enables >90% CM Induction

(A) Scheme for differentiation in bioreactors and microscopic assessment of aggregates over time. Scale bars, 200 μm.

(B) Growth kinetics in DASbox (n = 3 independent experiments) and the Bioblock system (n = 3 independent experiments). Error bars represent SEM.

(C) Cardiac differentiation efficiency assessed by FC on day 10 for cTNT, SA, and MHC revealed >90% purity in DASbox and ∼90% in Bioblock (n = 3 independent experiments). Error bars represent SEM.

(D) Correlation of ROR1+/CD13+ on day 3 and respective CM content on day 10 of Phoenix in specific differentiation conditions resulting from upscale experiments. r denotes Pearson's correlation coefficient (n = 28 in seven independent experiments). Dotted line denotes 95% confidence interval.

See also Figure S4.