Fig.3.

Longitudinal change in Aβ₄₀, Aβ₄₂, and AβOs in TBS, SDS, and FA fractions from Aβ+ or Aβ– treated mouse brains. Amounts of Aβ_x - 40 (A–C), Aβ_x - 42 (D–F), and AβOs (G–I) in TBS, SDS, and FA fractions of Aβ+ (red) and Aβ– (blue) immunized mouse brains measured using ELISA at 23, 43, and 59 weeks old. There were no significant differences in the levels of Aβ_x - 40 (A, B) and Aβ_x - 42 (D–F) between Aβ+ and Aβ– treated groups at any time points or in any fractions, except for the Aβ_x - 40 in the FA fraction at 59 weeks old (C: ^*p<0.05). Significant suppression of AβOs in TBS soluble fractions was revealed in Aβ+ treated mice compared with Aβ– treated mice (G and enlarged illustration, ^****p < 0.0001). Amounts of AβOs in SDS and FA fractions were very low compared with Aβ₄₀ and Aβ₄₂ in the same fraction (H, I). Mice at 23 weeks (n = 9 for Aβ+, n = 11 for Aβ–), 43 weeks (n = 7 for Aβ+, n = 6 for Aβ–), and 59 weeks (n = 6 for Aβ+, n = 7 for Aβ–) were measured by ELISA.