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Linear extrapolation of kinetic unfolding rate constants versus GdnHCl concentration for γSWT and its individual domains. All experiments consisted of 10 μg/mL protein concentration, 100 mM sodium phosphate, 1 mM EDTA, 5 mM DTT, pH 7.0 buffer, and defined concentrations of GdnHCl at 18°C. (A) γSWT, (B) γSN, and (C) γSC are shown.
