Table 1.
Equilibrium Unfolding and Refolding Parameters for γD- and γS-Crystallin Wild-Type and Isolated Domains at 18°C
| Protein | Equilibrium Transition 1 |
Equilibrium Transition 2 |
||||
|---|---|---|---|---|---|---|
| CMa |
Apparent m Valueb |
Apparent ΔG°N–I/Uc |
CMa |
Apparent m Valueb |
Apparent ΔG°N/I–Uc |
|
| 18°C | 18°C | |||||
| γDWT | 2.6 ± 0.035 | 4.0 ± 0.6 | 10.5 ± 1.5 | 3.5 ± 0.02 | 2.9 ± 0.03 | 10.1 ± 0.2 |
| γDN | 1.6 ± 0.08 | 3.5 ± 0.8 | 5.5 ± 1.3 | – | – | – |
| γDC | – | – | – | 3.2 ± 0.05 | 3.2 ± 0.3 | 10.4 ± 0.7 |
| γSWT | 2.7 ± 0.04 | 4.7 ± 0.7 | 12.6 ± 2.0 | – | – | – |
| γSN | 2.12 ± 0.03 | 3.3 ± 0.8 | 6.9 ± 1.7 | – | – | – |
| γSC | 2.7 ± 0.02 | 3.5 ± 0.4 | 9.6 ± 1.0 | – | – | – |
a
Transition midpoints in units of M GdnHCl.
b
Apparent m values in units of kcal∗mol−1∗M−1.
c
Free energy of unfolding in the absence of GdnHCl in units of kcal∗mol−1.