Table 1. Bioengineering S. cerevisiae for recombinant interfering RNA expression.

Recombinant Yeast Strains Generated	Resulting Advancements
Restoration of Dicer and Argonaut activity in S. cerevisiaea	Functional RNAi activity in yeast.
Generation of recombinant S. cerevisiae for delivery of shRNA targeting murine CD40b	Successful delivery of an shRNA expression system to murine intestinal DC cells, leading to successful gene silencing in mice.
S. cerevisiae expressing shRNA corresponding to mosquito genesc	Successful delivery of dried inactivated yeast interfering RNA pesticides to mosquitoes; shRNA expression cassette successfully integrated into yeast genome.
S. cerevisiae that express long dsRNA molecules targeting Drosophila crop pestsd	Development of S. cerevisiae system for expressing and delivering long dsRNA insecticides to crop pests
S. cerevisiae shRNA expression system for improvement of itaconic acid production straine	Optimization of a yeast strain as a metabolic engineering tool; evaluation of parameters that improve shRNA expression
RNAi screening libraries generatedf	Facilitated development of tunable yeast biomolecule production systems; evaluation of parameters that improve expression of long dsRNA molecules in S. cerevisiae

Key advancements resulting from the generation of yeast strains expressing recombinant interfering RNA molecules are noted here and discussed in further detail in this review. ^aReference [3]; ^bReference [13] ; ^cReferences [9, 10]} ; ^dReference [14]; ^eReference [15]; ^fReferences [16, 17].