Table 5.

In vitro and in vivo anthelmintic activity against Haemonchus contortus

Part A: In vitro	Concentration (μg/mL)
Egg hatch test ED50 ± SD	Methanolic extract	Aqueous extract
Mix1	539.2 ± 174.4	940.0 ± 73.0**
Mix2	486.9 ± 228.9	880.0 ± 67.8**
Larval development test LD50 ± SD
Mix1	266.6 ± 107.2	922.7 ± 232.1**
Mix2	826.4 ± 370.5	1256.5 ± 465.0**
Part B: In vivo	Numbers of adult worms
Mean number of adult worms ± SD	Infected animals	Infected animals with Mix1	Infected animals with Mix2
	1523 ± 187	1113 ± 274*	1327 ± 351

In vitro activity: data are means ± SDs of three independent assays with duplicate samples. Ovicidal and larvicidal activities are expressed as the concentration (μg/mL) of a median lethal dose (ED₅₀ and LD₅₀, respectively; the concentration of a methanolic or aqueous extract that prevents 50% of the eggs from hatching or larvae from developing to the infective L3 stage) using the egg hatch test or larval development test

In vivo activity: *Reduction of adult worms relative to infected animals 70 days after infection (P < 0.05)

**Means within a row are significantly different at P < 0.01