Fig. 1.

Constitutive knockout of microglial P2Y12R using P2Y12-floxed mice. a Schematic of the strategy used to generate the loxP flanked p2ry12 mice. b Representative immunostaining images showing P2Y12R was expressed in Cd11b + cells in WT, but absent in P2Y12^f/f:CX₃CR1^Cre/+ (constitutive KO) mice. Scale bar: 50 μm. c Open field test locomotor activity between constitutive microglial P2Y12R KO (n = 12) and WT control (n = 10) groups. d The pooled results from open field test indicated similar locomotor activity (left) and center area exploration (right) between WT and constitutive KO groups