Fig. 4.

Microglial P2Y12R deficiency enhances the excitability of the hippocampal CA1 pyramid neurons. a Representative firing patterns of CA1 pyramidal neurons in response to 500 ms, 50 pA current steps in constitutive microglial P2Y12R KO and WT mice. b Input/output curve displaying the number of action potentials as the input current increases for each group. c The voltage threshold of action potential firing was significantly reduced in P2Y12R constitutive KO mice compared with WT mice. d There is no difference in resting membrane potential between the two groups. e Representative spontaneous excitatory postsynaptic currents (sEPSCs) from both groups. f and g sEPSC amplitude (f) and frequency (g) for the indicated groups *p < 0.05, **p < 0.01, ***p < 0.001 (t-test), n = 11 neurons from 3 mice in constitutive KO group and n = 8 neurons from 3 mice in WT group. All data are presented as the mean ± SEM