Fig. 5.

Gln metabolism promotes cell viability and inhibits autophagy of estrogen-sensitive and insensitive UECC. a Different concentrations (0, 0.02, 0.2, 2, 20, 200 mmol/L) of Gln were used to treat Ishikawa and KLE, and then CCK-8 assay was used to analyze cell viability. b, c Ishikawa and KLE cells were incubated with Gln (200 mmol/L) and/or CB-839 (1 μmol/L) for 48 h, and then cell viability was evaluated by CCK-8 assay; d, e the expression of autophagy-related proteins (LC3B, Beclin-1 and p62) was evaluated by western blotting, and gray scale of LC3B, Beclin-1 and p62 was analyzed by image J software; f, g In addition, TEM was performed to test autophagic degree of Ishikawa and KLE cells after treatment with Gln and/or CB-839. The autophagic degree was presented as APs and ALs per visual field. The data are expressed as the mean ± SD. *P < 0.05, **P < 0.01 or ***P < 0.001 and ns means of no statistical significance. Ctrl: control group, G: Gln-treated group, C: CB-839-treated group, G + C: Gln plus CB-839-treated group