Fig. 2.

LINC00460 induced the EMT phenotype and was primarily localized in the cytoplasm. a-c The expression of some EMT markers (E-cadherin, N-cadherin, Vimentin, ZEB1 and ZEB2) was detected by Western blot analysis when LINC00460 was knocked down or overexpressed in HNSCC cells. d, e The expression of E-cadherin, N-cadherin, Vimentin, ZEB1 and ZEB2 was detected by qRT-PCR when LINC00460 was knocked down (d) or overexpressed (e) in HNSCC cells. f Cell nuclear/cytoplasmic fractionation and qRT-PCR showed the cellular distribution of LINC00460 in HN30 and SCC-9 cells. (NEAT1, TUG1, MALAT1, BIRC5, U6 and GAPDH were used as separation quality standards and endogenous controls). g FISH analysis of LINC00460 in CAL-27 cells. (The nuclei were stained with DAPI, and 18S rRNA was used as a cytoplasmic marker. Scale bar: 10 μm.) *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001