Fig. 5.

LINC00460 facilitated PRDX1 entry into the nucleus, and PRDX1 promoted the transcription of LINC00460 and EMT-related genes. a, b The expression of EMT markers (E-cadherin, N-cadherin, Vimentin, ZEB1 and ZEB2) was detected by Western blot analysis in CAL-27 cells when PRDX1 was knocked down or overexpressed. c, d The expression of EMT-associated genes (E-cadherin, N-cadherin, Vimentin, ZEB1 and ZEB2) was detected by qRT-PCR analysis in CAL-27 cells when PRDX1 was knocked down (c) or overexpressed (d). e qRT-PCR analysis of LINC00460 expression in CAL-27 cells when PRDX1 was knocked down or overexpressed. f The protein levels of PRDX1 in nuclear and cytoplasmic fractions were analyzed by Western blotting in CAL-27 cells transfected with SS-LINC00460 or LINC00460 vector. g ChIP-PCR analysis of anti-HA- or IgG- immunoprecipitated LINC00460 promoter fragments from CAL-27 cells stably transduced with PRDX1-HA. h ChIP-PCR analysis of anti-HA- or IgG- immunoprecipitated ZEB1, ZEB2 and VIM promoter fragments from CAL-27 cells stably transduced with PRDX1-HA when LINC00460 was overexpressed (LINC00460) or not overexpressed (NC). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns: no significance