Fig. 5.

Depletion of viromes at the species level after depletion of the gut bacterial composition validated by real-time PCR. (a) As the templates, the DNA and cDNA extracted from fecal samples were detected directly by real-time PCR. (b) The samples that were not detected directly from DNA or cDNA were subjected to MDA and then used as templates for detection by real-time PCR. We used the Ct numbers to show viral richness. The black line represents the mean of 3 replicates, and the red line represents the SEM. The samples that were not detected are not shown