Figure 3.

Intranasal SynGEM protein induces heterogeneous mucosal IgA responses. Subjects were given SynGEM or placebo and nasal wash IgA was measured by ELISA using unstabilized F protein as coating antigen at time points up to 180 days after “prime.” (A–C) Individuals in the placebo (A), low-dose (B), and high-dose (C) groups were clustered if they displayed a greater than twofold rise in nasal IgA titers according to the time point of maximal increase. (D–F) Cluster analysis of individual participant-level data was carried out by Fisher's exact test on placebo (D), low-dose (E), and high-dose (F) groups at the indicated time points after vaccination. **P < 0.01. Vaccinations are indicated by red triangles.