Table 2.
Flow cytometric analysis of cell cycle distribution in HeLa cells treated with GA (in %). The results are presented from 3 independent experiments as mean ± SD; significantly different, * P < 0.05, ** P < 0.01 versus untreated cells (control); + P < 0.05 versus GA. Sub-G0/G1 fraction of cells identified as apoptotic population
Treatment | Time (h) | sub-G0/G1 | G0/G1 | S | G2/M |
---|---|---|---|---|---|
Control | 24 | 1.50 ± 0.33 | 57.70 ± 1.54 | 20.80 ± 1.35 | 20.00 ± 1.11 |
48 | 1.80 ± 0.71 | 64.40 ± 1.88 | 17.40 ± 0.89 | 16.30 ± 0.61 | |
72 | 2.80 ± 0.25 | 69.20 ± 1.77 | 14.50 ± 1.20 | 13.60 ± 0.55 | |
NAC | 24 | 2.90 ± 1.10 | 58.90 ± 1.41 | 18.80 ± 0.99 | 19.40 ± 1.22 |
48 | 3.10 ± 0.77 | 67.50 ± 2.32 | 13.80 ± 0.57 | 15.60 ± 0.88 | |
72 | 2.70 ± 0.43 | 70.40 ± 1.19 | 12.40 ± 0.66 | 14.50 ± 0.67 | |
GA | 24 | 8.00 ± 1.30* | 50.00 ± 2.11* | 20.00 ± 1.23 | 22.00 ± 1.19 |
48 | 16.20 ± 1.20** | 46.50 ± 2.75** | 18.50 ± 1.70 | 18.90 ± 1.44 | |
72 | 20.90 ± 1.55** | 47.90 ± 1.35** | 16.50 ± 1.79 | 14.80 ± 1.30 | |
NAC/GA | 24 | 4.60 ± 0.21 | 60.70 ± 1.14+ | 18.20 ± 0.73 | 16.50 ± 1.13 |
48 | 7.30 ± 0.76*,+ | 60.90 ± 2.10+ | 14.30 ± 1.30 | 17.50 ± 1.90 | |
72 | 5.50 ± 1.25+ | 60.70 ± 1.93*,+ | 15.60 ± 0.56 | 18.20 ± 0.33 |