Table 3.
Annexin V/PI flow cytometry analysis of apoptosis occurrence in HeLa cells after GA treatment alone and after NAC pre-treatment (in %). The results are presented from 3 independent experiments. Significantly different, *P < 0.05, **P < 0.01, ***P < 0.001 versus untreated cells (control); +P < 0.05, ++P < 0.01 versus GA
| Treatment | Time (h) | An−/PI− Live cells |
An+/PI− Early apoptotic |
An+/PI+ Late apoptotic and death |
|---|---|---|---|---|
| Control | 24 | 90.90 ± 0.65 | 2.50 ± 0.16 | 6.57 ± 0.42 |
| 48 | 89.60 ± 1.11 | 2.60 ± 0.59 | 7.79 ± 1.23 | |
| 72 | 89.50 ± 1.99 | 3.17 ± 0.60 | 7.32 ± 0.81 | |
| NAC | 24 | 89.10 ± 1.66 | 3.50 ± 0.44 | 7.35 ± 0,66 |
| 48 | 92.30 ± 1.82 | 1.88 ± 0.33 | 5.77 ± 0.28 | |
| 72 | 94.80 ± 1.43 | 1.68 ± 0.19 | 3.56 ± 0.37 | |
| GA | 24 | 52.80 ± 2.45** | 22.70 ± 1.00** | 24.53 ± 3.29* |
| 48 | 30.30 ± 2.99*** | 31.30 ± 2.66** | 38.40 ± 2.45** | |
| 72 | 28.40 ± 1.75*** | 21.80 ± 2.12** | 49.78 ± 3.33*** | |
| NAC/GA | 24 | 72.90 ± 1.77*,++ | 16.00 ± 0.90*,+ | 11.11 ± 1.71*,+ |
| 48 | 68.30 ± 2.20**,++ | 13.60 ± 1.59*,+ | 18.14 ± 1.54*,++ | |
| 72 | 67.40 ± 3.19**,++ | 11.30 ± 0.69*,+ | 21.25 ± 2.34*,++ |