Fig. 5.

AtPARP3 is incapable of binding to the substrate NAD⁺. a Immunoblotting with streptavidin/HRP. b Immunoblotting with anti-His antibody. The purified proteins were blotted onto an activated PVDF membrane and then incubated with 25 μM biotinylated NAD⁺ in the presence of 500 nM broken DNA for 30 min at 25 °C. The NAD⁺ binding proteins were detected by blotting with streptavidin/HRP. An anti-His antibody was used to visualize the loading amounts of the proteins blotted on the membrane