Figure 1|.

Schematic overview of the protocol for derivation of mature kidney glomerular podocytes from human iPS cells. The diagram shows sequential differentiation stages in the protocol. The concentrations of growth factors and signaling molecules are shown. Also shown are the markers used to characterize the cells at each stage of the differentiation protocol. Black square indicates a pause point, where the protocol may be paused and the intermediate mesoderm cells could be stored by cryopreservation. GSC, goosecoid; WT1, Wilm’s tumor 1; OSR1, odd-skipped related transcription factor 1; BMP-7, bone morphogenetic protein 7; VEGF, vascular endothelial growth factor; ECM, extracellular matrix. Figure modified with permission from Reference 5.