Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Aug 5;2019:8390585. doi: 10.1155/2019/8390585

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2019 E. S. Ershova et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Determination of rDNA CN in human cfDNA using NQH. (a) Scheme of the human ribosomal repeat. Segment of rDNA analyzed with NQH is shown. (b) (1) Photo of the membrane fragments with visualized rDNA. Three spots are applied for each DNA sample. The calibration DNA samples containing 640 (control 1) or 420 (control 2) copies of rDNA were put onto these spots of the filter. (2) The filter was scanned and the average integral intensity I (±SD) of the spots was determined with software application “Imager 6.0.” (c) Dependence of I on the total cfDNA concentration in the sample plotted for the standard samples (dashed lines) and for the tested DNA samples 1-9 (as an example). (2) The ratio I _i/I _control 2 was calculated. (d) Calibration dependence of rDNA CN on the I _i/I _control 2 ratio plotted for DNA calibration samples with known rDNA CN. (2) The rDNA CN in the cfDNA was calculated.