Figure 5.
The activation of the MAPK pathway. The effect of H2S on MAPK activation in chicken spleens. (a) and (b) represent the effect of H2S on the mRNA (a) and phosphorylated protein (b) expressions of JNK, ERK, and p38 in chicken spleens. The results are from at least three independent experiments. Data are represented as the means ± SD (n = 10). β-Actin and total JNK, total ERK, and total p38 were selected as the reference of mRNA and phosphorylated protein expressions, respectively. ∗ shows significant difference from the corresponding control groups (p < 0.05); ∗∗ shows significant difference from the corresponding control groups (p < 0.01).