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. 2019 Jul 29;2019:9164202. doi: 10.1155/2019/9164202

Figure 3.

Figure 3

Functional analysis of purified CL-12. Binding of purified CL-12 (6.5 μg/ml) to A. fumigatus was assessed by flow cytometry (a) or fluorescence microscopy (scale bar, 100 μm; 40x magnification) (b). CL-12-induced agglutination was assessed by a change of forward and side scatter morphology and shown in the inset in (a). (c) Dose-dependent binding of purified CL-12 to A. fumigatus. (d, e) Amplification of complement activation by purified CL-12. A. fumigatus were incubated with purified CL-12 (6.5 μg/ml) prior to inducing complement activation by serumMBL- in the presence of 5 mM Mg2+/10 mM EGTA, and then C3b (d) or TCC (e) deposition was analyzed. The mean fluorescence intensity (MFI) was used to assess protein binding. The results are expressed as the mean ± SEM of at least three independent experiments. ∗P < 0.01.