Table 1: Summary of conditions tested.

Summary of the acoustic conditions tested for ultrasound-mediated Caco-2 permeabilization with PCCAs, including the number of experimental replicates performed.

Condition	PCCA (Y/N)	Pressure (kPa)	Cycles (#)	Dextran size (kDa)	Experimental replicates
1	Y	300	20	70	3
2		400			3
3		500			3
4		600			3
5	Y	300	40	70	6
6		400			3
7		500			3
8		600			3
9 - sham	N	NA	NA	70	3
10 - US only	N	600	40	70	3
11 - PCCA only	Y	NA	NA	70	3

Abbreviations: PCCA – phase change contrast agent; kPa – kilopascal; kDa – kilodalton; US – ultrasound; Y – yes; N – no; NA – not applicable