Fig. 5.

Surface plasmon resonance sensograms of hPXR interaction with PB. BIACORE SPR affinity assays were carried out to measure the comparative binding kinetics of RIF (A, positive control) and PB (B) to hPXR protein as described in Materials and Methods. Measurements of comparative binding kinetics of CITCO (C) and PB (D) to hCAR protein were used as positive and negative controls, respectively. Sensorgrams of the interaction generated by the instrument were analyzed by the software BIAeval 3.2.