Table 1. Comparison of different clearing methods in composition and clearance time.

Method	Solutions	Time needed
ScaleA2	4M Urea, 10% (wt/vol) glycerol, 0.1% Triton X-100	> 2 days
Sucrose	15, 30, 45, 60% sucrose + 2% Triton X-100	4 days
ClearT	20%, 40%, 80%, and 95% formamide solutions in PBS (v/v)	30 min