Fig. 2.

Effects of point mutants in the allosteric binding pocket on sensitivity to the antagonist AZ11645373. Effects of point mutations on AZ11645373 sensitivity are reported by their pIC₅₀ value. Black dotted lines correspond to a 3-fold change in sensitivity. Pink residues are variant, green have similar properties, and those in black are conserved between P2X and P2X7Rs. Exact values for each receptor tested are given in Supplemental Table 32. *P < 0.05; ***P < 0.001; ****P < 0.0001, n ≥ 3.