Table 2.
Effects of OA and OA-NLC on Ca2+ mobilization in the absence or presence of albumin
| Without albumin | With albumin | |||
|---|---|---|---|---|
| Peak [Ca2+]i (nM) | t1/2 (sec) | Peak [Ca2+]i (nM) | t1/2 (sec) | |
| Control | 297.34 ± 12.86 | 25.85 ± 2.92 | 346.40 ± 11.43 | 22.84 ± 0.94 |
| OA | 298.17 ± 17.62 | 6.90 ± 0.09*** | 334.62 ± 5.12 | 23.57 ± 0.87 |
| OA-NLC | 325.38 ± 10.05 | 9.40 ± 0.25*** | 371.04 ± 10.68 | 6.53 ± 0.41*** |
Notes: In the absence of albumin, neither OA nor OA-NLC affected peak [Ca2+]i values in activated neutrophils; however, they significantly decreased t1/2. In the presence of albumin, OA-NLC, but not OA, affected t1/2 values. ***P<0.001, as compared to the control assay.
Abbreviations: t1/2, the time required for [Ca2+]i to return to half of the peak value; OA, oleic acid; OA-NLC, oleic acid within nanostructured lipid carriers.