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Representative western blot showing that expression of FLAG-tagged mutants JMJD6-L90A/K91A/R95A and JMJD6-L90A to N96A in cells stable expressing shRNA against Jmjd6 (shJ6) was detected only immediately after transduction and selection. Expression of the JMJD6 wildtype and the iron binding mutant H187A/D189A are shown as controls. β-ACTIN levels were monitored as a loading control.
