Fig 6. The JMJD6 AT hook-like domain mutant cannot co-activate expression of the genes encoding the adipogenic regulators, PPARγ2 and C/EBPα and does not bind to the genes encoding those regulators.

(A) Analysis of mRNA levels of the adipocyte lineage-determinant genes, Cebpα and Pparγ2 in control cells and in JMJD6 knockdown cells (shJ6) expressing wild type JMJD6 or the JMJD6-Polyserine stretch or JMJD6-AT hook-like mutants or the empty vector that were differentiated for 72h. The individual mRNA levels were normalized to Eef1a1 mRNA levels. The normalized expression levels of shRNA scramble control cells were set as 1. (B) ChIP analysis of JMJD6 binding to the Pparγ2 and Cebpα loci in control cells or JMJD6 knockdown cells (shJ6) expressing the empty vector, wild type JMJD6 or the JMJD6-AT hook-like mutant that were differentiated for 24h. Data represent average values +/- standard deviation from three independent experiments. *P<0.05, **P<0.01.