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. 2019 Aug 20;14(8):e0216015. doi: 10.1371/journal.pone.0216015

Table 1. Oligonucleotides for plasmid construction.

Primer name Sequence 5’ to 3’
shScr forward GATCCTGGCGGCGAGTGAAGTACGTGATAAGTGTGCTGTCCTTATCACGTACTTCACTCGCCGCCATTTTTGGAAA
shScr reverse AGCTTTTCCAAAAATGGCGGCGAGTGAAGTACGTGATAAGGACAGCACACTTATCACGTACTTCACTCGCCGCCAGG
shJ6-3 forward GATCCCGCTGACACCCAGAGAACAAGTGTGCTGTCCTTGTTCTCTGGGTGTCAGCTTTTTGGAAA
shJ6-3 reverse AGCTTTTCCAAAAAGCTGACACCCAGAGAACAAGGACAGCACACTTGTTCTCTGGGTGTCAGCGG
pBABE-JMJD6 forward CCCGGATCCCTCACGATGAACCACAAGAGCAAG
pBABE-JMJD6 reverse CGGTCGACTTATCATTTGTCATCGTCGTCCTTGTAGTCCCTGGAGGAGCTGCGCTC
JMJD6 H187A/D189A forward GCGTTCTGGAACTGGGATTGCCATCGCCCCTCTGGGG
JMJD6 H187/AD189A reverse CCCCAGAGGGGCGATGGCAATCCCAGTTCCAGAACGC
JMJD6 H273A forward CAGGGGGCTGGTGGGCTGTTGTCCTCAACC
JMJD6 H273A reverse GGTTGAGGACAACAGCCCACCAGCCCCCTG
JMJD6 T184A Forward CCACCGCGTTCTGGAGCTGGGATTCACATCG
JMJD6 T184A Reverse CGATGTGAATCCCAGCTCCAGAACGCGGTGG
JMJD6 N197A forward GGGACCAGTGCCTGGGCTGCCTTAGTTCAGGG
JMJD6 N197A reverse CCCTGAACTAAGGCAGCCCAGGCACTGGTCCC
JMJD6 K204A forward CTTAGTTCAGGGTCACGCGCGGTGGTGCCTCTTC
JMJD6 K204A reverse GAAGAGGCACCACCGCGCGTGACCCTGAACTAAG
JMJD6 T285A forward CACCACCATTGCCATCGCCCAGAACTTTGCCAG
JMJD6 T285A reverse CTGGCAAAGTTCTGGGCGATGGCAATGGTGGTG
JMJD6 K317A forward GAAGTGGTATAGGATCTTGGCACAGGAGCACCCTGAGCTG
JMJD6 K317A reverse CAGCTCAGGGTGCTCCTGTGCCAAGATCCTATACCACTTC
JMJD6-Poly-Serine forward CTCTGACAGCTCCAGCGACGCTGCTGCCGCCGCTGCTTCCAGCTCGTCAGA
JMJD6 Poly-Serine reverse TCTGACGAGCTGGAACTAGCGGCGGCAGCAGCGTCGCTGGAGCTGTCAGAG
JMJD6 AT hook-like forward CTTCCCTGTTGTGTGGCACAAGACGGTAGCAGCGGCAGCAGCGTTATCAAGGAAGTGGTATAGGATCTTG
JMJD6 AT hook-like reverse CAAGATCCTATACCACTTCCTTGATAACGCTGCTGCCGCTGCTACCGTCTTGTGCCACACAACAGGGAAG
JMJD6 Δ Nterm forward CCCGGATCCCTCACGATGGATAGCAGCTATGGCGAACACCC
JMJD6 Δ Nterm reverse Same as pBABE-JMJD6 reverse
JMJD6 Δ Cterm forward Same as pBABE-JMJD6 forward
JMJD6 Δ Cterm reverse CGGTCGACTTATCATTTGTCATCGTCGTCCTTGTAGTCCCTGCGCCGCTTTTTTCGGCGTGGTCTCCCTCTTACCGTCTTGTGC
J6 Δ JmjC_PCR1 forward Same as pBABE-JMJD6 forward
J6 Δ JmjC_PCR1 reverse CAAGATCCTATACCACTTCCTTGATAACTTTGGGTGTTCGCCATAGCTGCTATCG
J6 Δ JmjC_PCR2 forward Product of J6 Δ JmjC_PCR1 (Megaprimer)
J6 Δ JmjC_PCR2 reverse Same as pBABE-JMJD6 reverse