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. 2019 Aug 20;14(8):e0221431. doi: 10.1371/journal.pone.0221431

Fig 2. Sub-inhibitory polymyxin B concentration inhibits V. cholerae A1552 and O139 biofilm formation at the early adhesion stage.

Fig 2

(A) and (B) Biofilm quantification and (C) planktonic optical density of A1552 and MO10 strain cultures after 24h incubation. (A) Polymyxin B (PmB) or water (control) were added from the beginning, and (B) at one hour (1h) or two hours (2h) after culture starts. A1552 without (-) PmB (white) and A1552 with (+) 25 μg/mL PmB (black); MO10 without (-) PmB (blue) and MO10 with (+) 25 μg/mL PmB (red). Biofilm formation kinetics of A1552 (C) and MO10 (D), PmB is added at the beginning of culture. (A, B, C and D) All results were done in technical duplicates within biological triplicates. Data correspond to mean ± SD. (A and B) Two-Way ANOVA with Tukey correction was performed to compare each condition (ns; * P < 0.01; ** P < 0.005).