Fig 2. Immunohistochemical validation of subcellular localization.
Paraffin sections double-immunostained for Rpl22 (HA) and RFP-NLS (A) and Argonaute2 (V5) and RFP-NLS (B) confirming separation of Tagger components. HA, hemagglutinin; RFP-NLS, red fluorescent protein-nuclear localization signal; PCL, principal cell layer of the hippocampus; Rpl22, large subunit ribosomal protein 22; WT, wild-type.