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. 2019 Aug;60(10):3584–3594. doi: 10.1167/iovs.19-27197

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RGC-derived CASK is not required for RGC survival. (A) Toluidine blue staining shows no reduction in ON size in Cask^fl/fl::Calb2-Cre^+/+ compared to Cask^fl/fl. Scale bar: 50 μm. (B) Quantification of cross-sectional area in Cask^fl/fl::Calb2-Cre^+/+ compared to Cask^fl/fl. (n = 4 mice per genotype). (C) No reduction in number of cells in GCL layer using RBPMS (RNA-binding protein with multiple splicing) or NeuroTrace. Scale bar: 50 μm. (D) Quantification of number of cells in GCL using RBPMS in Cask^fl/fl::Calb2-Cre^+/+ compared to Cask^fl/fl. (n = 10 mice per genotype). (E) Quantification of number of cells in GCL using NeuroTrace in Cask^fl/fl::Calb2-Cre^+/+ compared to Cask^fl/fl. (n = 6 mice per genotype). (F) TEM of RGC axons in the ON in Cask^fl/fl::Calb2-Cre^+/+ compared to Cask^fl/fl. Scale bar: 5 μm. (G) Quantification of density of axons per image in Cask^fl/fl::Calb2-Cre^+/+ compared to Cask^fl/fl. (n = 4 mice per genotype). (H–J). Analysis of axon area in Cask^fl/fl::Calb2-Cre^+/+ ON compared to Cask^fl/fl. Quantification of the average size of fine axons (I) and course axons (J). For all panels data are plotted as mean ± SEM.