Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Antibody | anti-Mcm2-7 | Bell Lab | UM174 | 1:10,000 (rabbit polyclonal) |
| Antibody | anti-Cdc45 | Lõoke et al., 2017 | HM7135 | 1:2000 (rabbit polyclonal) |
| Antibody | anti-GINS | Lõoke et al., 2017 | HM7128 | 1:2000 (rabbit polyclonal) |
| Antibody | anti-Mcm10 | Lõoke et al., 2017 | HM7602 | 1:2000 (rabbit polyclonal) |
| Antibody | anti-cMyc agarose | Sigma | Sigma: A7470 | |
| Antibody | anti-V5 agarose | Sigma | Sigma: A7345 | |
| Chemical compound, drug | DY549-P1-maleimide | Dyomics | Dyomics: 549P1-03 | |
| Chemical compound, drug | DY649-P1-maleimide | Dyomics | Dyomics: 649P1-03 | |
| Chemical compound, drug | SYBR Gold nucleic acid stain | Invitrogen | Invitrogen: S11494 | |
| Commercial assay or kit | illustra MicroSpin G-50 columns | GE Healthcare | GE Healthcare: 27533001 | |
| Gene (Saccharomyces cerevisiae) | MCM2 | Saccharomyces Genome Database | SGD: S000000119 | |
| Gene (Saccharomyces cerevisiae) | MCM3 | Saccharomyces Genome Database | SGD: S000000758 | |
| Gene (Saccharomyces cerevisiae) | MCM4 | Saccharomyces Genome Database | SGD: S000006223 | |
| Gene (Saccharomyces cerevisiae) | MCM5 | Saccharomyces Genome Database | SGD: S000004264 | |
| Gene (Saccharomyces cerevisiae) | MCM6 | Saccharomyces Genome Database | SGD: S000003169 | |
| Gene (Saccharomyces cerevisiae) | MCM7 | Saccharomyces Genome Database | SGD: S000000406 | |
| Other | TransFluoSpheres Streptavidin-Labeled Microspheres | Thermo Fisher Scientific | Thermo Fisher: T10711 | |
| Other | Amersham Hybond-XL | GE Healthcare | GE Healthcare: RPN303S | Membrane for imaging replication assay |
| Peptide, recombinant protein | DNase I | Worthington | Worthington: LS006333 | |
| Peptide, recombinant protein | Proteinase K | Thermo Fisher Scientific | Thermo Fisher: AM2548 | |
| Peptide, recombinant protein | Nb.BsrDI | New England Biolabs | New England Biolabs: R0648S | |
| Peptide, recombinant protein | Peptide for coupling to dyes for Sortase labeling | Ticau et al., 2015 | NH2-CHHHHHHHHHHLPETG | |
| Sequence-based reagent | oligo for 1.3 kb template | IDT | 5'-Biotin-GATCGGTGCGGGCCTCTTCGC-3' | |
| Sequence-based reagent | oligo for 1.3 kb unlabeled template | IDT | 5'-GGAAAGCGGGCAGTGAGCGC-3' | |
| Sequence-based reagent | oligo for 1.3 kb fluorescently-labeled template | IDT | 5'-Alexa488-GGAAAGCGGGCAGTGAGCGC-3' | |
| Software, algorithm | Matlab | Mathworks |