Extended Data Figure 1.
AOA reprograms TH17 cell differentiation toward ITreg cells. a. Screening procedure showing how the screening was conducted. b. Effects of AOA on TH17 cell differentiation. c, The effect of AOA on mTOR, AMPK, and c-Myc. Differentiating TH17 cells (with or without 0.75 mM AOA) were collected for analyzing p-AMPK1a, mTOR downstream signaling proteins, S6K, 4E-BP1 or c-Myc. d, The effect of AOA on the proliferation of TH17 cells. CD4 naive T cells were labeled with cell tracer violet, and then cultured under TH17 condition or ITreg condition (the same condition as TH17 condition except without IL-1β, IL-6 and IL-23). At the end of experiment, the cells were analyzed by intracellular staining. e) AOA promoted ITreg cell differentiation. f) AOA did not affect the survival of TH17 cell culture. The cells were differentiated under TH17 condition with or without AOA. At the end of differentiation, the cells were collected for analysis immediately after 7-AAD was added into cell culture.