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. Author manuscript; available in PMC: 2020 Oct 1.
Published in final edited form as: Brain Res. 2019 Jun 29;1720:146311. doi: 10.1016/j.brainres.2019.146311

Figure 3. Effects of ERα or ERβ Blockade on VMN GABAergic Nerve Cell ERα, ERβ, and GPER Protein Expression in Hypoglycemic in Male versus Female Rats.

Figure 3.

VMN GAD65/67-ir neurons were evaluated by Western blot for ERα [Panel A; male data at left, female data at right], ERβ [Panel B; male data at left, female data at right], and GPER [Panel C; male data at left, female data at right] protein expression in vehicle-pretreated euglycemic controls (solid white bars; n=6 males, n=6 females) controls and vehicle- (solid gray bars; n=6 males, n=6 females), MPP- (diagonal-striped bars; n=6 males, n=6 females), or PHTPP (cross-hatched gray bars; n=6 males, n=6 females)-pretreated INS (10.0 U/kg bw, sc) - injected animals. Data depict mean normalized protein O.D. measures ± S.E.M. Data were analyzed by two-way ANOVA for sex versus treatment. *p <0.05; **p <0.01; ***p <0.001.