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Nanoparticles adhere and penetrate through the mature biofilm. Representative 3D-image of 48-hour old MRSA biofilm (blue) grown in an 8-well chamber slide treated with 0.5 mg/ml blank-np (green). The polysaccharide component of EPS was stained with concanavalin A-405. The images were acquired using Leica SP5 confocal laser scanning microscope and the image was constructed using Volocity software v6.3. The scale bars represent 100 μm. The experiment was performed twice.
