Skip to main content
. Author manuscript; available in PMC: 2020 Aug 19.
Published in final edited form as: Dev Cell. 2019 Jul 25;50(4):436–446.e5. doi: 10.1016/j.devcel.2019.06.013

Figure 3. Loss of AP2 adaptor in AAGAB KO cells.

Figure 3.

(A) Diagram of the GST pull-down assay measuring AAGAB binding to the α subunit (trunk domain, a.a. 1-621) of AP2 adaptor. (B) Left: Coomassie blue-stained gel showing the binding of His6-SUMO-AAGAB to GST-tagged α subunit from GST pull-down assays. Right: Coomassie blue-stained gel showing His6-SUMO-AAGAB proteins precipitated by nickel beads. AAGAB expression levels were much higher than GST-α-subunit such that the latter was often not evident in nickel bead pull-down samples. (C) Representative confocal microscopy images showing the α subunit of AP2 adaptor in WT or mutant HeLa cells. Bar: 10 μm. (D) Representative TIRF microscopy images showing the α subunit of AP2 adaptor in WT or mutant HeLa cells. Bar: 10 μm. (E) Immunoblots showing the expression levels of the indicated proteins in WT or AAGAB KO HeLa cells.