Skip to main content
. Author manuscript; available in PMC: 2020 Aug 20.
Published in final edited form as: Acc Chem Res. 2019 Aug 5;52(8):2301–2308. doi: 10.1021/acs.accounts.9b00250

Figure 3.

Figure 3.

CP sequesters Fe(II) under aerobic conditions and shifts Fe redox equilibrium to favor Fe(II).22 (A) Depletion of Fe from Tris:TSB medium (62:38 20 mM Tris, 100 mM NaCl, pH 7.5:TSB medium) supplemented with 2 mM Ca(II) by 10.5 μM CP-Ser in the absence or presence of ~3 mM β-mercaptoethanol (BME). The mean and SDM are reported (n = 3). (B & C) Fe(III) citrate (10 mM) was incubated with 10.5 μM (B) CP-Ser or (C) ΔΔ variant in the presence of 2 mM Ca(II) and the Fe redox speciation was monitored by the ferrozine assay (75 mM HEPES, 100 mM NaCl, pH 7.0 at 30 °C, 150 rpm).22 The mean and SDM are reported (n = 6). Panels B and C were reproduced with permission from ref. 22. Copyright 2017 the Royal Society of Chemistry.