Figure 4:

Workflow for Preparation of FFPE Tissues for Glycan Analysis by MALDI IMS. FFPE tissue blocks are sliced into 5 ?m sections and placed onto glass slides. Once sectioned slides are prepared, they are placed at 60°C for 1 hour. Next, slides are placed through a series of deparaffinization washes and antigen retrieval. PNGaseF enzyme is then applied in a fine mist to the slides using a HTX TMSprayer to cleave glycans on the slides. Slides are then incubated at 37°C for 2 hours. After incubation, a MALDI compatible matrix is applied. At the conclusion of this process, slides can be stored under vacuum until ready for MALDI IMS analysis.