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TIRF images of nanobubble formation and dissolution on MAA-co-MMA surface. Images were acquired during the following sequence (note: all liquids contain AlexFluor488): rinse with ethanol (A), exchange to air-saturated water (B), exchange with ethanol and exchange to degassed water (C), exchange with ethanol and finally exchange with air-saturated water (D). Scale bar = 10 μm for all images.
