Figure 2.

a) Schematic showing the RBC phantoms. b) Photograph showing a RBC phantom. c) 5x optical microscope image of the RBCs encapsulated in agarose phantoms. d) Close-up optical microscope image of the RBC phantom taken at 40x magnification showing that the encapsulated RBCs showed preserved morphology. e) Schematic representation of the HIFU treatment of the RBC phantoms. f) Photograph of a HIFU treated RBC phantom containing PL-hMSN (200 μg mL⁻¹) for 30 s at a pulse duration of 16.8 μs, a pulse repetition frequency of 10 Hz (duty cycle of 0.017%), and peak negative pressure of 16.8 MPa. g) Close-up image of the RBC phantom in (f) showing the nine ablated regions. h) and i) Photographs of a sliced RBC phantom containing PL-hMSN (200 μg mL⁻¹) after HIFU treatment showing the cross-sectional appearance of three ablated regions. j) and k) Optical microscope images of an ablated region. l) Damaged areas and ablation area fractions in the RBC phantoms containing or not containing nanoparticles (200 μg mL⁻¹) after HIFU treatment for 30 s at a pulse duration of 16.8 μs and peak negative pressure of 16.8 MPa. Error bars = 1 SD, studies were run in triplicate.