REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Custom made secondary antibodies (rabbit & mouse)	MRC Phosphorylation and Ubiquitylation unit, University of Dundee	N/A
α-Acetyl lysine	Cytoskeleton	Cat#AAC03
α-Alpha tubulin	Santa-Cruz	Cat#sc-8035
α-ANXA1	Sigma	Cat#HPA011272
α-Beta actin	Sigma	Cat#A5441
α-OXPHOS	Abcam	Cat#ab110413
α-PGK1	Abcam	Cat#ab199438
α-PGM1	Protein Tech	Cat#15161-1-AP
α-PKM1	Protein Tech	Cat#15821-1-AP
Chemicals, Peptides, and Recombinant Proteins
13C labeled nucleotides, nucleosides	Trammell and Brenner, 2013	N/A
18O- nicotinamide	Kolodziejska-Huben et al., 2002	N/A
18O nicotinamide riboside	Yang et al., 2007	N/A
2-mercaptoethanol	VWR	Cat#441435C
Acetonitrile, Optima LC/MS	Fisher Scientific	Cat#A955-4
Adenosine 5′-triphosphate disodium salt hydrate	Sigma-Aldrich	Cat#A2383
Adenosine 5′diphosphate	Sigma-Aldrich	Cat#A5285
Adenosine diphosphate	Sigma-Aldrich	Cat#1905
Adenosine monophosphate	Sigma-Aldrich	Cat#A2252
ADPR	Sigma-Aldrich	Cat#A0752
Ammonium acetate	Sigma-Aldrich	Cat#238074
Antimycin A	Sigma-Aldrich	Cat#A8674
Benazmidine	Millipore	Cat#S7124222
Bovine serum albumin	Sigma	Cat#A2153
BSA, essentially fatty acid free	Sigma-Aldrich	Cat#A6003
Calcium carbonate	Sigma-Aldrich	Cat#C4830
Catalase from bovine liver	Sigma-Aldrich	Cat#C9322
Chemiluminescent HRP substrate	Millipore	Cat#WBKLS0500
Cytidine	Sigma-Aldrich	Cat#C122106
Cytochrome c	Sigma-Aldrich	Cat#C7752
d3, 18O methyl nicotinamide	Trammell et al., 2016a	N/A
d4-nicotinic acid	CDN isotopes	Cat#D-4368
Dithiotreitol	Sigma-Aldrich	Cat#D0632
DL-octanoyl carnitine-HCl	Tocris bioscience	Cat#605
Dnase I, Rnase-free	Thermo Scientific	Cat#EN0521
EDTA	Sigma	Cat#E1644
EGTA	Sigma-Aldrich	Cat#E4378
EGTA	Sigma	Cat#E4378
FCCP	Sigma-Aldrich	Cat#2920
Glucose reagent	Werfen Ltd	Cat#00018250740
Glutamic acid monosodium salt hydrate	Sigma-Aldrich	Cat#G1626
Glycerol reagent	Randox	Cat#GY105
Glycine	VWR	Cat#0167
HEPES	Fluka	Cat#BP310-1
Imidazole	Sigma-Aldrich	Cat#56750
Inosine	Sigma-Aldrich	Cat#I4125
Inosine monophosphate	Sigma-Aldrich	Cat#57510
Lactate Reagent	Randox	Cat#LC2389
Lactobionic acid	Sigma-Aldrich	Cat#153516
LDS sample buffer	Invitrogen	Cat#NP0008
Magnesium chloride hexahydrate	Sigma-Aldrich	Cat#M2670
Malic acid	Sigma-Aldrich	Cat#M1000
MES Free Acid Hydrate	Sigma-Aldrich	Cat#M8250
N1-methyl-2-pyridone-5-carboxamide	TLC Pharmaceutical Standards	Cat#N-0621
N1-methyl-4-pyridone-3-carboxamide	TLC Pharmaceutical Standards	Cat#N-0627
NAAD	Sigma-Aldrich	Cat#N4256
NAD	Sigma-Aldrich	Cat#N0632
NADP	Sigma-Aldrich	Cat#N5755
NAR	gift	N/A
N-d3 methyl −4 pyridone-3-carboxamide	TLC Pharmaceutical Standards	Cat#N-0628
NEFA reagent	Randox	Cat#FA115
Nicotinamide	Sigma-Aldrich	Cat#72340
nicotinamide N-oxide	Sigma-Aldrich	Cat#N3258
Nicotinamide riboside	ChromaDex	Cat#ASB-00014315
Nicotinic acid	Sigma-Aldrich	Cat#N4126
N-methyl nicotinamide	Cayman Chemical	Cat#16604
NMN	Sigma-Aldrich	Cat#N3501
Phosphocreatine disodium salt hydrate	Sigma-Aldrich	Cat#P7936
Potassium phosphate monobasic	Sigma-Aldrich	Cat#P9791
Protease inhibitor cocktail	Roche	Cat#11873580001
Rotenone	Sigma-Aldrich	Cat#R8875
Saponin from Quillaja bark	Sigma-Aldrich	Cat#S7900
SDS	ITW	Cat#A1112
Skimmed milk powder	Cell Signaling	Cat#9999
Sodium chloride	VWR	Cat#27800.360
Sodium fluoride	Alfa Aesar	Cat#7681-49-4
Sodium orthovanodate	Aldrich	Cat#450243
Sodium pyrophosphate	Sigma	Cat#221368
Sodium succinate dibasic hexahydrate	Sigma-Aldrich	Cat#S2378
Sucrose	Sigma-Aldrich	Cat#S9378
Sucrose	VWR	Cat#0335
Taurine	Sigma-Aldrich	Cat#T8691
TRI Reagent	Sigma-Aldrich	Cat#T9414
Triglyceride reagent	Werfen Ltd	Cat#00018258740
Tris-base	Fisher	Cat#BP152
Tris-HCL	Fisher	Cat#BP153
Triton X-100	Sigma	Cat#101634725
Tween-20	VWR	Cat#0777
U-13C6 glucose	Cambridge Isotope	Cat#CLM-1396-pk
Water, Optima LC/MS	Fisher Scientific	Cat#W6-4
β-Glycerophosphate	Sigma	Cat#G9422
Critical Commercial Assays
Citrate synthase assay kit	Sigma-Aldrich	Cat#CS0720
Coomassie protein assay reagent	Thermo Fisher	Cat#1856209
Insulin assay	Mercodia	Cat#10-1113-01
Deposited Data
Raw and processed data files for RNA sequencing	This paper	GEO: GSE133261
Oligonucleotides
Please refer to Table S6	This paper	N/A
Other
Precast gels	BioRad	Cat#5671084
Thermo hypercarb 2.1 × 100 mm column, 3μm	Fisher	Cat#35003-102130