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. 2019 Aug 20;9:12109. doi: 10.1038/s41598-019-48601-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression levels of 11 putative ripening-associated DzDofs under three different ripening treatments: natural (control), ethylene-induced, and 1-MCP-delayed ripening. Gene transcript level was quantified using qRT-PCR. Elongation factor 1 alpha (EF-1α) was used as the reference gene to normalize the expression levels of each other gene. Error bars indicate ± standard deviations (SD). For each DzDof, comparisons were made of its expression levels among three different ripening treatments. Three independent biological replicates were used. Three technical replicates were also performed for each biological replicate. Bars with different letters above them were significantly different according to Duncan’s multiple range test (P < 0.05). The DzDofs marked with asterisks (*) indicate the seven confirmed ripening-associated DzDofs.