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. 2019 Aug 20;9:12133. doi: 10.1038/s41598-019-48546-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Using the eDNA Index, the biomass-index correlation coefficient (ρ) by amplification efficiency for each amplified taxon. Those taxa with a relative amplification efficiency >= 0.6 have particularly strong correlations (median ρ = 0.96). As shown by shading, the eDNA Index behaves similarly for species with greater and lesser proportions of biomass in the community. Simulated biomass varied over two orders of magnitude across taxa; averaging across time-points narrows this range to a factor of two, and the relative mean biomass expressed here reflects that smaller range.