Table 1.
Contents and concentration of growth factors and cell culture supplements used in each AT2 organoid culture condition
| Final working concentration | C1 | C2 | C3 | C4 | C5 | C6 | C7 | C8 | C9 | C10 | C11 | C12 | C1+M | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| SAGM | – | + | + | + | + | + | + | + | + | + | + | + | – | + |
| ADMEM/F12 | – | – | – | – | – | – | – | – | – | – | – | – | + | – |
| KGF | 20 ng/mL | + | + | + | + | + | + | + | + | + | + | + | + | + |
| EGF | 50 ng/mL | + | + | + | + | + | + | + | + | + | + | + | + | + |
| A83-01 | 1 μM | – | + | + | + | + | + | + | + | + | + | + | + | – |
| Rspo1 | 200 ng/mL | – | + | + | + | + | + | – | + | + | + | – | + | – |
| Wnt3a | 100 ng/mL | – | + | + | + | + | – | – | + | + | – | + | + | – |
| Y-27532 | 5 μM | – | + | + | + | – | – | – | + | – | + | + | + | – |
| Noggin | 100 ng/mL | – | + | + | – | – | – | – | – | + | + | + | + | – |
| FGF10 | 100 ng/mL | – | + | – | – | – | – | – | + | + | + | + | + | – |
| B27 | 1× | – | – | – | – | – | – | – | – | – | – | – | + | – |
| N2 | 1× | – | – | – | – | – | – | – | – | – | – | – | + | – |
| HEPES | 10 mM | – | – | – | – | – | – | – | – | – | – | – | + | – |
| Gentamycin | 1× | – | – | – | – | – | – | - | – | – | – | – | + | – |
| P/S | 1× | – | – | – | – | – | – | – | – | – | – | – | + | – |
| Lung mesenchyme | 26,000 cells | – | – | – | – | – | - | – | – | – | – | – | – | + |